high throughput rtpcr instrument biomark hd  (fluidigm)

Journal: Frontiers in Veterinary Science

Article Title: Design of a High-Throughput Real-Time PCR System for Detection of Bovine Respiratory and Enteric Pathogens

doi: 10.3389/fvets.2021.677993

Figure Lengend Snippet: Sensitivity and amplification efficiency of rtPCR assays on the Rotor-Gene Q and BioMark platforms.

Article Snippet: The 192.24.DA IFC chip was placed in the IFC controller RX (Fluidigm) for loading and mixing for approximately 30 min and then subject to thermal cycling in the high-throughput rtPCR instrument BioMark HD (Fluidigm) with the following cycle conditions: 50°C for 2 min, 95°C for 10 min, followed by 40 cycles of 95°C for 15 s and 60°C for 60 s. Samples were tested in single reactions, and the assays were performed in duplicates.

Techniques: Amplification, Reverse Transcription Polymerase Chain Reaction

Journal: Frontiers in Veterinary Science

Article Title: Design of a High-Throughput Real-Time PCR System for Detection of Bovine Respiratory and Enteric Pathogens

doi: 10.3389/fvets.2021.677993

Figure Lengend Snippet: Feces and nasal swab samples analyzed on the high-throughput rtPCR system.

Article Snippet: The 192.24.DA IFC chip was placed in the IFC controller RX (Fluidigm) for loading and mixing for approximately 30 min and then subject to thermal cycling in the high-throughput rtPCR instrument BioMark HD (Fluidigm) with the following cycle conditions: 50°C for 2 min, 95°C for 10 min, followed by 40 cycles of 95°C for 15 s and 60°C for 60 s. Samples were tested in single reactions, and the assays were performed in duplicates.

Techniques: Reverse Transcription Polymerase Chain Reaction

Journal: Frontiers in Veterinary Science

Article Title: Design of a High-Throughput Real-Time PCR System for Detection of Bovine Respiratory and Enteric Pathogens

doi: 10.3389/fvets.2021.677993

Figure Lengend Snippet: Test of repeatability of the rtPCR assays on the high-throughput rtPCR platform.

Article Snippet: The 192.24.DA IFC chip was placed in the IFC controller RX (Fluidigm) for loading and mixing for approximately 30 min and then subject to thermal cycling in the high-throughput rtPCR instrument BioMark HD (Fluidigm) with the following cycle conditions: 50°C for 2 min, 95°C for 10 min, followed by 40 cycles of 95°C for 15 s and 60°C for 60 s. Samples were tested in single reactions, and the assays were performed in duplicates.

Techniques: Reverse Transcription Polymerase Chain Reaction, Standard Deviation

Journal: Frontiers in Veterinary Science

Article Title: Design of a High-Throughput Real-Time PCR System for Detection of Bovine Respiratory and Enteric Pathogens

doi: 10.3389/fvets.2021.677993

Figure Lengend Snippet: Sensitivity and amplification efficiency of rtPCR assays on the Rotor-Gene Q and BioMark platforms.

Article Snippet: The repeatability of the rtPCR assays on the high-throughput rtPCR platform (BioMark HD) was evaluated by testing the positive controls in 13 separate chip runs.

Techniques: Amplification, Reverse Transcription Polymerase Chain Reaction

Journal: Frontiers in Veterinary Science

Article Title: Design of a High-Throughput Real-Time PCR System for Detection of Bovine Respiratory and Enteric Pathogens

doi: 10.3389/fvets.2021.677993

Figure Lengend Snippet: Heat map showing the specificity of the rtPCR assays on the high-throughput rtPCR system by testing known positive controls. To the left: rtPCR assays . At the top: the positive controls and a no-template control (NTC). Each square corresponds to a single rtPCR reaction. Cq values for each reaction are indicated by color; the corresponding color scale is presented in the legend on the right. A black square is considered a negative result. A black X is shown if the amplification curve deviates too much from an ideal amplification curve.

Article Snippet: The repeatability of the rtPCR assays on the high-throughput rtPCR platform (BioMark HD) was evaluated by testing the positive controls in 13 separate chip runs.

Techniques: Reverse Transcription Polymerase Chain Reaction, High Throughput Screening Assay, Control, Amplification

Journal: Frontiers in Veterinary Science

Article Title: Design of a High-Throughput Real-Time PCR System for Detection of Bovine Respiratory and Enteric Pathogens

doi: 10.3389/fvets.2021.677993

Figure Lengend Snippet: Feces and nasal swab samples analyzed on the high-throughput rtPCR system.

Article Snippet: The repeatability of the rtPCR assays on the high-throughput rtPCR platform (BioMark HD) was evaluated by testing the positive controls in 13 separate chip runs.

Techniques: Reverse Transcription Polymerase Chain Reaction

Journal: Frontiers in Veterinary Science

Article Title: Design of a High-Throughput Real-Time PCR System for Detection of Bovine Respiratory and Enteric Pathogens

doi: 10.3389/fvets.2021.677993

Figure Lengend Snippet: Test of repeatability of the rtPCR assays on the high-throughput rtPCR platform.

Article Snippet: The repeatability of the rtPCR assays on the high-throughput rtPCR platform (BioMark HD) was evaluated by testing the positive controls in 13 separate chip runs.

Techniques: Reverse Transcription Polymerase Chain Reaction, Control, Standard Deviation

Journal: Frontiers in Veterinary Science

Article Title: Estimating Clinically Relevant Cut-Off Values for a High-Throughput Quantitative Real-Time PCR Detecting Bacterial Respiratory Pathogens in Cattle

doi: 10.3389/fvets.2021.674771

Figure Lengend Snippet: Results of the mixed-effects model for analysis of variables associated with respiratory disease in calves including analysis of the best rtPCR Cq cut-off differentiating between sick and healthy calves for P. multocida, M. bovis, H. somni, M. haemolytica and T. pyogenes .

Article Snippet: The objective was to develop and illustrate a field-data driven statistical method to guide the selection of relevant quantification cycle cut-off values for pathogens associated with BRD for the high-throughput rtPCR system “Fluidigm BioMark HD” based on nasal swabs from calves.

Techniques: Reverse Transcription Polymerase Chain Reaction

Journal: Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc

Article Title: Development of a high-throughput real-time PCR system for detection of enzootic pathogens in pigs

doi: 10.1177/1040638719890863

Figure Lengend Snippet: Heat map showing the specificity of the real-time PCR (rtPCR) assays included on the 48.48DA by testing known positive controls. At the top: rtPCR assays (Tables 1, ​,2).2). To the left: the positive controls, a no-template control (NTC), a negative cDNA/pre-amplification control, and a negative pre-amplification control. Each square corresponds to a single rtPCR reaction. Cycle threshold (Ct) values for each reaction are indicated by color; the corresponding color scale is presented in the legend on the right. A black square is considered a negative result. A black X is shown if the amplification curve deviates too much from an ideal amplification curve.

Article Snippet: To mitigate these restrictions, rtPCR assays have been optimized for the high-throughput rtPCR BioMark platform (Fluidigm).

Techniques: Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Control, Amplification